Human CLEC-2A Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB72191
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 705529
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC-2A
Trp49-Leu174 (predicted)
Accession # Q6UVW9
Trp49-Leu174 (predicted)
Accession # Q6UVW9
Specificity
Detects human CLEC-2A in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity
with recombinant human (rh) CLEC-2D, rhCLEC-2, or rhCD69 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CLEC-2A Antibody
Detection of Human CLEC-2A by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CLEC-2A at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CLEC-2A in Human Skin.
CLEC-2A was detected in immersion fixed paraffin-embedded sections of human skin using Mouse Anti-Human CLEC-2A Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB72191) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human CLEC-2A Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line
Sample: U937 human histiocytic lymphoma cell line, Jurkat human acute T cell leukemia cell line, and Bowes human melanoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CLEC-2A
Long Name
C-type Lectin Domain Family 2, Member A
Alternate Names
CLEC2A, KACL, PILAR
Entrez Gene IDs
387836 (Human)
Gene Symbol
CLEC2A
UniProt
Additional CLEC-2A Products
Product Documents for Human CLEC-2A Antibody
Product Specific Notices for Human CLEC-2A Antibody
For research use only
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