Human CLEC4D/CLECSF8 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2806

R&D Systems, part of Bio-Techne
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AF2806
AF2806-SP
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CLEC4D/CLECSF8 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CLEC4D/CLECSF8
Gly52-Asn215
Accession # Q8WXI8

Specificity

Detects human CLEC4D/CLECSF8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) CLECSF9 and rhCLEC4F is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human CLEC4D/CLECSF8 Antibody

Detection of CLEC4D/CLECSF8 antibody in Human Monocytes antibody by Flow Cytometry.

Detection of CLEC4D/CLECSF8 in Human Monocytes by Flow Cytometry.

Human whole blood monocytes were stained with Goat Anti-Human CLEC4D/CLECSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2806, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Applications for Human CLEC4D/CLECSF8 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human whole blood monocytes

Western Blot

0.1 µg/mL
Sample: Recombinant Human CLEC4D/CLECSF8
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4D/CLECSF8

Human CLEC4D, also known as MCL and CLECSF8, is a 30 kDa type II transmembrane (TM) glycoprotein that belongs to the CLR (C-type Lectin Receptor) family of molecules. It is synthesized as a 215 amino acid (aa) protein that contains a 17 aa N-terminal cytoplasmic domain, a 21 aa TM segment, and a 177 aa C-terminal extracellular region. The extracellular region shows a short stalk and a 118 aa CRD (carbohydrate recognition domain). The nature of its carbohydrate ligand is unknown. CLEC4F is restricted to monocytes/macrophages and serves as an endocytic receptor. Homodimers and homotrimers form on the cell surface. The human CLEC4D extracellular region shares 63% aa sequence identity with the mouse extracellular region.

Long Name

C-type Lectin Domain Family 4, Member D

Alternate Names

CD368, CLEC6, CLECSF8, Mpcl

Entrez Gene IDs

338339 (Human); 17474 (Mouse)

Gene Symbol

CLEC4D

UniProt

Q8WXI8

Additional CLEC4D/CLECSF8 Products

Product Documents for Human CLEC4D/CLECSF8 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CLEC4D/CLECSF8 Antibody

For research use only

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