Human Collybistin/ARHGEF9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7848
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 809309
Product Specifications
Immunogen
E. coli-derived recombinant human Collybistin/ARHGEF9
Asn390-Lys516
Accession # O43307
Asn390-Lys516
Accession # O43307
Specificity
Detects human Collybistin/ARHGEF9 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Collybistin/ARHGEF9 Antibody
Detection of Human and Mouse Collybistin/ARHGEF9 by Western Blot.
Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Collybistin/ARHGEF9 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Collybistin/ARHGEF9 in Human Brain.
Collybistin/ARHGEF9 was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human Collybistin/ARHGEF9 Monoclonal Antibody (Catalog # MAB7848) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Collybistin/ARHGEF9 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla)
Western Blot
2 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue
Sample: Neuro‑2A mouse neuroblastoma cell line, human brain (cortex) tissue, and mouse brain (cortex) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Collybistin/ARHGEF9
Long Name
Cdc42 Guanine Nucleotide Exchange Factor (GEF) 9
Alternate Names
ARHGEF9, KIAA0424, PEM-2
Gene Symbol
ARHGEF9
UniProt
Additional Collybistin/ARHGEF9 Products
Product Documents for Human Collybistin/ARHGEF9 Antibody
Product Specific Notices for Human Collybistin/ARHGEF9 Antibody
For research use only
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