Human Copeptin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6077
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 579021
Product Specifications
Immunogen
Peptide corresponding to the human Copeptin precursor
Ala126-Gly140
Accession # P01185
Ala126-Gly140
Accession # P01185
Specificity
Detects human Copeptin in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Copeptin Antibody
Detection of Human Copeptin by Western Blot.
Western blot shows lysates of human kidney (cortex) tissue and human kidney (medulla) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Copeptin Monoclonal Antibody (Catalog # MAB6077) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Copeptin at approximately 7-10 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Copeptin Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human kidney (cortex) tissue and human kidney (medulla) tissue under non-reducing conditions only
Sample: Human kidney (cortex) tissue and human kidney (medulla) tissue under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Copeptin
Copeptin is a 39 amino acid glycosylated peptide that is a proteolytic product derived from a precursor which also contains Vasopressin and Neurophysin 2 peptides. The precursor protein is synthesized and cleaved in the hypothalamus before transport to the pituitary for storage and release. Copeptin is secreted in equimolar amounts with Vasopressin. It is more stable than Vasopressin and serves as a surrogate indicator of Vasopressin release. Vasopressin plays a major role in blood pressure regulation through control of water retention in the kidney and vascular tone. Serum levels of Copeptin are associated with metabolic syndrome, insulin resistance, sepsis, and heart dysfunction following myocardial infarction. Human Copeptin shares 79% sequence identity with mouse and rat Copeptin.
Alternate Names
ADH;ARVP;AVP;AVP-NPII;AVRP;Vasopressin-neurophysin 2-copeptin;VP
Entrez Gene IDs
551 (Human)
Gene Symbol
AVP
UniProt
Additional Copeptin Products
Product Documents for Human Copeptin Antibody
Product Specific Notices for Human Copeptin Antibody
For research use only
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