| Specificity: |
Detects a synthetic peptide corresponding to residues surrounding amino acid 501 of human CPT1A protein in direct ELISA.
Applications for Human CPT1A Antibody
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Recommended Concentration
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Sample |
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Immunohistochemistry |
3-25 µg/mL |
Immersion fixed paraffin-embedded sections of human kidney |
Scientific Data Examples for Human CPT1A Antibody
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Immunohistochemistry
Detection of CPT1A in Human Kidney.
CPT1A was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human CPT1A Monoclonal Antibody (Catalog # MAB11570) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Formulation, Preparation and Storage
| Reconstitution |
Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.
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| Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
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| Stability & Storage |
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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Reconstitution Calculator
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Product Documents for Human CPT1A Antibody
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