Human CRHBP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2796
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CRHBP
Tyr25-Leu322
Accession # P24387.2
Tyr25-Leu322
Accession # P24387.2
Specificity
Detects human CRHBP in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse CRHBP is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human CRHBP Antibody
Detection of CRHBP in Human Liver Cancer.
CRHBP was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Goat Anti-Human CRHBP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2796) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human CRHBP Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Liver Cancer
Sample: Immersion fixed paraffin-embedded sections of Human Liver Cancer
Western Blot
0.1 µg/mL
Sample: Recombinant Human CRHBP
Sample: Recombinant Human CRHBP
Reviewed Applications
Read 4 reviews rated 3.8 using AF2796 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRHBP
Long Name
Corticotropin Releasing Hormone Binding Protein
Alternate Names
CRFBP
Entrez Gene IDs
1393 (Human)
Gene Symbol
CRHBP
UniProt
Additional CRHBP Products
Product Documents for Human CRHBP Antibody
Product Specific Notices for Human CRHBP Antibody
For research use only
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