NS0 cell line transfected with human CX3CL1/Fractalkine was stained with Mouse Anti-Human CX3CL1/Fractalkine Monoclonal Antibody (Catalog # MAB3652R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin. View our protocol for Staining Intracellular Proteins.

Chemotaxis Induced by CX3CL1/Fractalkine and Neutralization by Human CX3CL1/Fractalkine Antibody.

Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND₅₀ is typically 1-5 µg/mL.

Applications for Human CX3CL1/Fractalkine Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: NS0 Cell Line Transfected with Human CX3CL1/Fractalkine fixed with paraformaldehyde and permeabilized with saponin

Neutralization

Measured by its ability to neutralize CX3CL1/Fractalkine-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CX3CR1. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 100 ng/mL Recombinant Human CX3CL1/Fractalkine.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

CX3CL1, also known as Fractalkine, is a type I membrane protein in which a chemokine domain possessing a unique C-X3-C cysteine motif is tethered on a long mucin-like stalk. It can also be released as a soluble molecule upon proteolysis at a membrane proximal site.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423

Additional CX3CL1/Fractalkine Products

Product Documents for Human CX3CL1/Fractalkine Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CX3CL1/Fractalkine Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CX3CL1/Fractalkine Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3652R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CX3CL1/Fractalkine Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CX3CL1/Fractalkine Antibody

Detection of CX3CL1/Fractalkine in HEK293 Human Cell Line Transfected with Human CX3CL1/Fractalkine and eGFP by Flow Cytometry.