Human CXCL13/BLC/BCA-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF801
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque), Rabbit, Xenograft
Applications
Validated:
Dual RNAscope ISH-IHC Compatible, Immunohistochemistry, Neutralization, Western Blot
Cited:
Bioassay, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human CXCL13/BCA-1
Val23-Arg94
Accession # Q53X90
Val23-Arg94
Accession # Q53X90
Specificity
Detects CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CXCL13/BLC/BCA-1 Antibody
Chemotaxis Induced by CXCL13/BLC/BCA-1 and Neutralization by Human CXCL13/BLC/BCA-1 Antibody.
Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CXCL13/BLC/BCA-1 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801). The ND50 is typically 1-4 µg/mL.
CXCL13/BLC/BCA‑1 in Human Lymphoma.
CXCL13/BLC/BCA-1 was detected in immersion fixed paraffin-embedded sections of human lymphoma using Goat Anti-Human CXCL13/BLC/BCA-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF801) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of CXCL13/BLC/BCA-1 in Human Lymphoma.
Formalin-fixed paraffin-embedded tissue sections of Hodgkin’s Lymphoma were probed for CXCL13 mRNA (ACD RNAScope Probe, catalog # 311321; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using goat anti-human CXCL13 polyclonal antibody (R&D Systems catalog # AF801) at 5ug/mL with 1-hour incubation at room temperature followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm.Applications for Human CXCL13/BLC/BCA-1 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human lymphoma
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil and human lymphoma
Sample: Immersion fixed paraffin-embedded sections of human tonsil and human lymphoma
Western Blot
0.1 µg/mL
Sample: Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX)
Sample: Recombinant Human CXCL13/BLC/BCA-1 (Catalog # 801-CX)
Neutralization
Measured by its ability to neutralize CXCL13/BLC/BCA-1-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CXCR5. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 50 ng/mL Recombinant Human CXCL13/BLC/BCA-1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 7 reviews rated 4.6 using AF801 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL13/BLC/BCA-1
References
- Gunn, M.D. et al. (1998) Nature, 391:799.
- Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
- Forster, R. et al. (1996) Cell 87:1037.
Alternate Names
ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13
Gene Symbol
CXCL13
UniProt
Additional CXCL13/BLC/BCA-1 Products
Product Documents for Human CXCL13/BLC/BCA-1 Antibody
Product Specific Notices for Human CXCL13/BLC/BCA-1 Antibody
For research use only
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