HEK293 human embryonic kidney cell line transfected with (A) human CXCR3 or (B) irrelevant transfectants and eGFP was stained with and either Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Flow Cytometry Isotype Control (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

CXCR3 in Human Tonsil.

CXCR3 was detected in perfusion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to infiltrating lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Chemotaxis Induced by CXCL11/I-TAC and Neutralization by Human CXCR3 Antibody.

Recombinant Human CXCL11/I-TAC (Catalog # 672-IT) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL11/I-TAC (7 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR3 Monoclonal Antibody (Catalog # MAB160R). The ND₅₀ is typically 0.3-1.5 µg/mL.

Applications for Human CXCR3 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line Transfected with human CXCR3 and eGFP

Immunohistochemistry

0.5-25 µg/mL
Sample:

Perfusion fixed paraffin-embedded sections of human tonsil

Neutralization

Measured by its ability to neutralize CXCL11/I‑TAC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 7 ng/mL Recombinant Human CXCL11/I‑TAC.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR3

CXCR3 is a G protein-coupled chemokine receptor that binds the alpha chemokines MIG (CXCL9), IP-10 (CXCL10), and I-TAC (CXCL11). CXCR3 is expressed on activated T cells, B cells, and NK cells.

Alternate Names

CD183, CXCR3, GPR9

Entrez Gene IDs

2833 (Human); 12766 (Mouse); 84475 (Rat)

Gene Symbol

CXCR3

UniProt

P49682

Additional CXCR3 Products

Product Documents for Human CXCR3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CXCR3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CXCR3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB160R

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all CXCR3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human CXCR3 Antibody

Detection of CXCR3 in HEK293 Human Cell Line Transfected with human CXCR3 and eGFP by Flow Cytometry.