Human peripheral blood mononuclear cells (PBMCs) either (A) treated with 20 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) for 6 days or (B) untreated were stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Detection of CXCR6 in HEK293 Human cell line transfected with Human CXCR6 and eGFP by Flow Cytometry.

HEK293 Human cell line transfected with either (A) Human CXCR6 or (B) irrelevant transfectant and eGFP, was stained with Mouse Anti-Human CXCR6 Monoclonal Antibody (Catalog # MAB699) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

Detection of Human CXCR6 by Immunocytochemistry/Immunofluorescence

CXCR6 expression in tissues samples from LuCa patients(A) Representative images for CXCR6 tissue expression in lung tissues from non-neoplastic (n = 8), adenocarcinoma (n = 54) and squamous cell carcinoma (n = 24) were stained with isotype control or anti-CXCR6 antibodies. Brown (DAB) color shows CXCR6 staining. Images were captured using tissuefaxs cell analysis system from Tissuegnostics. (B) Immuno-intensities of CXCR6 were quantified using image analysis Aperio ImageScope v.6.25 software using ImageScope algorithm. ***p ≤ 0.0001 when compared between groups. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.3194), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human CXCR6 Antibody

Application

Recommended Usage

CyTOF-reported

Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human IL‑2 (Catalog # 202-IL) and HEK293 human cell line transfected with human CXCR6

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCR6

CXCR6, also known as Bonzo and STRL33, is a G protein coupled chemokine receptor expressed on activated T cells and tumor-infiltrating lymphocytes. It binds soluble CXCL16 and membrane-anchored CXCL16 expressed on dendritic cells and macrophages.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Entrez Gene IDs

10663 (Human); 80901 (Mouse); 100124593 (Rat)

Gene Symbol

CXCR6

UniProt

O00574

Additional CXCR6 Products

Product Documents for Human CXCR6 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human CXCR6 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human CXCR6 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB699

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all CXCR6 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human CXCR6 Antibody

Detection of CXCR6 in Human PBMCs by Flow Cytometry.