Human Cyclin D3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6570
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 588726
Product Specifications
Immunogen
E.coli-derived recombinant human Cyclin D3
Met1-Leu292
Accession # P30281
Met1-Leu292
Accession # P30281
Specificity
Detects human Cyclin D3 in direct ELISAs.
In direct ELISAs and Western blots,
approximately 5% cross-reactivity with recombinant human (rh) Cyclin B1 and D1
is observed. No cross-reactivity with rhCyclin A1, A2, B2, C, D2, E1, or E2 is
observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Cyclin D3 Antibody
Detection of Human and Mouse Cyclin D3 by Western Blot.
Western blot shows lysates of BaF3 mouse pro-B cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human Cyclin D3 Monoclonal Antibody (Catalog # MAB6570) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cyclin D3 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclin D3 in Human Breast Cancer Tissue.
Cyclin D3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human Cyclin D3 Monoclonal Antibody (Catalog # MAB6570) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cyclin D3 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human breast cancer tissue
Western Blot
1 µg/mL
Sample: BaF3 mouse pro-B cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Sample: BaF3 mouse pro-B cell line, Jurkat human acute T cell leukemia cell line, and K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB6570 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclin D3
Additional Cyclin D3 Products
Product Documents for Human Cyclin D3 Antibody
Product Specific Notices for Human Cyclin D3 Antibody
For research use only
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