Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5546
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 638708
Product Specifications
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
Glu2-Leu295
Accession # P50225
Glu2-Leu295
Accession # P50225
Specificity
Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SULT1A3 and rhSULT1C4 and approximately 10% cross-reactivity with rhSULT1B1 is observed. No cross-reactivity with rhSULT1E1, 2A1, 2B1, or 4A1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
Detection of Human Cytosolic Sulfotransferase 1A1/SULT1A1 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 1A1/SULT1A1 in Human Brain.
Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain
Sample: Immersion fixed paraffin-embedded sections of human brain
Immunoprecipitation
25 µg/mL
Sample: Cell lysates spiked with Recombinant Human SULT1A1 (Catalog # 5546-ST), see our available Western blot detection antibodies
Sample: Cell lysates spiked with Recombinant Human SULT1A1 (Catalog # 5546-ST), see our available Western blot detection antibodies
Western Blot
0.5 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 1A1/SULT1A1
Cytosolic Sulfotransferase 1A1 (SULT1A1; also phenol sulfotransferase 1 and thermostable phenol sulfotransferase) is a 35 kDa enzyme belonging to the sulfotransferase 1 family of proteins. Human SULT1A1 is 295 amino acids (aa) in length. Human SULT1A1 shares 78% and 73% aa identity with rat and mouse SULT1A1, respectively. SULT1A1 exists as a homodimer and is expressed in liver, lung, adrenal gland, brain, platelets, and skin. Functionally, it catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitter. It also mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could participate as a modulating factor of cancer risk.
Alternate Names
P-PST, PST, ST1A3, STP1, SULT1A1, TSPST1
Gene Symbol
SULT1A1
UniProt
Additional Cytosolic Sulfotransferase 1A1/SULT1A1 Products
Product Documents for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
Product Specific Notices for Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody
For research use only
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