Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5828
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 656229
Product Specifications
Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 2A1/SULT2A1
Ser2-Glu285
Accession # NP_003158
Ser2-Glu285
Accession # NP_003158
Specificity
Detects human Cytosolic Sulfotransferase 2A1/SULT2A1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human SULT1A1, 1A3, 4A1, 1B1, 2B1, or 1E1 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Detection of Human Cytosolic Sulfotransferase 2A1/SULT2A1 by Western Blot.
Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Mono-clonal Antibody (Catalog # MAB5828) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 2A1/SULT2A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cytosolic Sulfotransferase 2A1/SULT2A1 in HepG2 Human Cell Line.
Cytosolic Sulfotransferase 2A1/SULT2A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Cytosolic Sulfotransferase 2A1/ SULT2A1 Monoclonal Antibody (Catalog # MAB5828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line
Western Blot
2 µg/mL
Sample: Human liver tissue
Sample: Human liver tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cytosolic Sulfotransferase 2A1/SULT2A1
Sulfotransferase 2A1 (SULT2A1), also called DHEA-ST (DHEA/bile acid sulfotransferase), is a 35 kDa member of the cytosolic sulfotransferase family, which are Phase II drug-metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates. SULT2A1 is primarily expressed in liver and adrenal tissues. It sulfates steroids and bile acids, playing roles in maintaining cholesterol and bile acid homeostasis by increasing cholesterol catabolism and preventing toxicity from bile acid accumulation. Human SULT2A1 shares 63-65% amino acid identity with mouse and rat SULT2A1.
Alternate Names
DHEA-ST, DHEAS, hSTa, ST2A3, SULT2A1
Gene Symbol
SULT2A1
UniProt
Additional Cytosolic Sulfotransferase 2A1/SULT2A1 Products
Product Documents for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
Product Specific Notices for Human Cytosolic Sulfotransferase 2A1/SULT2A1 Antibody
For research use only
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