Human DC-SCRIPT/ZNF366 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4707
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human DC-SCRIPT/ZNF366
Met559-Ile744
Accession # Q8N895
Met559-Ile744
Accession # Q8N895
Specificity
Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human DC-SCRIPT/ZNF366 Antibody
Detection of DC-SCRIPT/ZNF366 in Human Mature Dendritic Cells by Flow Cytometry.
Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC-SCRIPT/ZNF366 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human DC-SCRIPT/ZNF366 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human monocyte-derived dentritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM‑CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB), then fixed with paraformaldehyde and permeabilized saponin
Sample: Human monocyte-derived dentritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM‑CSF (Catalog # 215-GM), LPS, Recombinant Human TNF‑ alpha (Catalog # 210-TA), and Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB), then fixed with paraformaldehyde and permeabilized saponin
Western Blot
0.1 µg/mL
Sample: Recombinant Human DC-SCRIPT/ZNF366
Sample: Recombinant Human DC-SCRIPT/ZNF366
Reviewed Applications
Read 1 review rated 4 using AF4707 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DC-SCRIPT/ZNF366
DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85 kDa member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255‑556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.
Long Name
Dendritic Cell-specific Transcript
Alternate Names
DCSCRIPT, ZNF366
Gene Symbol
ZNF366
UniProt
Additional DC-SCRIPT/ZNF366 Products
Product Documents for Human DC-SCRIPT/ZNF366 Antibody
Product Specific Notices for Human DC-SCRIPT/ZNF366 Antibody
For research use only
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