Human peripheral blood monocytes were stained with Mouse Anti-Human DEC-205/CD205 Monoclonal Antibody (Catalog # MAB2047, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).

Applications for Human DEC-205/CD205 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: Human peripheral blood monocytes

Reviewed Applications

Read 1 review rated 4 using MAB2047 in the following applications:

ELISA (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.

Alternate Names

CD205, DEC205, Ly75

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Gene Symbol

LY75

UniProt

O60449

Additional DEC-205/CD205 Products

Product Documents for Human DEC-205/CD205 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DEC-205/CD205 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human DEC-205/CD205 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2047

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all DEC-205/CD205 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DEC-205/CD205 Antibody

Detection of DEC-205/CD205 in Human Monocytes by Flow Cytometry.