Human DFF45/ICAD Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4679
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 495403
Product Specifications
Immunogen
E. coli-derived recombinant human DFF45/ICAD
Met1-Thr331 (predicted)
Accession # O00273
Met1-Thr331 (predicted)
Accession # O00273
Specificity
Detects human DFF45/ICAD in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human DFF45/ICAD Antibody
Detection of Human DFF45/ICAD by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A549 human lung carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human DFF45/ICAD Monoclonal Antibody (Catalog # MAB4679) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DFF45/ICAD at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group2.Applications for Human DFF45/ICAD Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A549 human lung carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: DFF45/ICAD
DFF45 (DNA fragmentation factor 45 kDa subunit, also known as ICAD) is a 45 kDa member of the CIDE domain-containing family of proteins. Human DFF45 is 331 amino acids (aa) in length. It contains an N-terminal CIDE domain (aa 17‑96) plus two caspase-3 cleavage sites (aa’s 117‑118 and 224‑225). DFF45 is a cytoplasmic protein that inhibits DFF40 DNAase. Normally, DFF45 and DFF40 form an enzymatically-inactive noncovalent heterodimer. Upon activation of the apoptotic cascade, DFF45 is cleaved into three fragments. These dissociate from DFF40 and induce DFF40 oligomerization and activation. Human DFF45 has two potential splice forms. One is truncated, and shows a seven aa substitution for aa 262‑331; a second is extended, and shows a 45 aa substitution for the C-terminal five amino acids. Human DFF45 is 76% aa identical to mouse DFF45.
Long Name
DNA Fragmentation Factor, Subunit alpha
Alternate Names
DFF1, DFFA, ICAD
Gene Symbol
DFFA
UniProt
Additional DFF45/ICAD Products
Product Documents for Human DFF45/ICAD Antibody
Product Specific Notices for Human DFF45/ICAD Antibody
For research use only
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