Human DIAPH-2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6806

R&D Systems, part of Bio-Techne
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MAB6806
MAB6806-SP
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 699142

View all DIAPH-2 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DIAPH-2
Val314-Gln540
Accession # O60879

Specificity

Detects human DIAPH-2 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human DIAPH-2 Antibody

Detection of Human DIAPH-2 antibody by Western Blot.

Detection of Human DIAPH-2 by Western Blot.

Western blot shows lysates of human testis tissue and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human DIAPH-2 Monoclonal Antibody (Catalog # MAB6806) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DIAPH-2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human DIAPH-2 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human testis tissue and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DIAPH-2

DIAPH2 (diaphenous homolog 2), also called DRF2 (diaphanous-related formin 2), is an 1101 amino acid (aa), 126 kDa (calculated) cytoplasmic protein in the Diaphenous family of actin binding proteins. Disruption of DIAPH2 has been correlated with premature ovarian failure in humans. Within the region used as an immunogen, human DIAPH2 shares 89% and 90% aa identity with mouse and rat DIAPH2, respectively. Two additional isoforms have been described. One, called hDia2C (1097 aa), is lacking aa 45-55, but has 7 aa inserted after aa 149. This form specifically binds RhoD and restricts movement of early endosomes along actin filaments. A 1106 aa form diverges after aa 1080.

Long Name

Diaphanous Homolog 2

Alternate Names

DIA2, DIAPH2, DRF2, POF2

Entrez Gene IDs

1730 (Human); 54004 (Mouse); 317189 (Rat)

Gene Symbol

DIAPH2

UniProt

O60879

Additional DIAPH-2 Products

Product Documents for Human DIAPH-2 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human DIAPH-2 Antibody

For research use only

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