HUVEC human umbilical vein endothelial cells were stained with Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')₂Secondary Antibody (Catalog # F0105B).

DLL4 in HUVEC Human Cells.

DLL4 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human DLL4 Monoclonal Antibody (Catalog # MAB1506) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human DLL4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: HUVEC human umbilical vein endothelial cells

Immunocytochemistry

8-25 µg/mL
Sample:

Immersion fixed HUVEC human umbilical vein endothelial cells

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using MAB1506 in the following applications:

Immunocytochemistry/Immunofluorescence (2 Reviews)
Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL4

Delta-like ligand 4 (DLL4) is a transmembrane protein that contains one DSL domain and eight tandem EGF-like repeats. DLL4 is expressed on arterial endothelial cells where it signals through Notch 1 and Notch 4. DLL4 expression is induced by VEGF and regulates the proliferation of endothelial tip cells during vascular sprouting. DLL4 blockade as well as overexpression can suppress tumor growth by promoting dysregulated angiogenesis. Within the extracellular domain, human DLL4 shares 85% amino acid sequence identity with mouse and rat DLL4.

Long Name

Delta-like 4

Alternate Names

delta 4, Delta 4 precursor, delta ligand 4, Delta4, delta-like 4 (Drosophila), delta-like 4 homolog, delta-like 4 homolog (Drosophila), delta-like 4 protein, delta-like protein 4, Drosophila Delta homolog 4, hdelta2, MGC126344, notch ligand delta-2, notch ligand DLL4

Entrez Gene IDs

54567 (Human); 54485 (Mouse); 100152163 (Porcine)

Gene Symbol

DLL4

UniProt

Q9NR61

Additional DLL4 Products

Product Documents for Human DLL4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human DLL4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human DLL4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB1506

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all DLL4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human DLL4 Antibody

Detection of DLL4 in HUVEC Human Cells by Flow Cytometry.