Human Drebrin 1 Antibody

Name: Human Drebrin 1 Antibody
Brand: R&D Systems
SKU: MAB7739
Rating: 4 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB7739

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 838102

View all Drebrin 1 Antibodies »

Product Summary for Human Drebrin 1 Antibody

Immunogen

E. coli-derived recombinant human Drebrin 1
Asn482-Asp649 (Ser553Pro)
Accession # Q16643

Specificity

Detects human Drebrin 1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Drebrin 1 Antibody

Detection of Human Drebrin 1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Drebrin 1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Drebrin 1 in HeLa Human Cell Line.

Drebrin 1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membrane and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Drebrin 1 by Simple Western^TM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.5 mg/mL. A specific band was detected for Drebrin 1 at approximately 162 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Drebrin 1 Monoclonal Antibody (Catalog # MAB7739) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Drebrin 1 Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Jurkat human acute T cell leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB7739 in the following applications:

Immunocytochemistry/Immunofluorescence (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Drebrin 1

Drebrin 1 (DBN-1 [developmentally-regulated brain protein1]; also drebrin-E/E2 [Embryonic]) is an intracellular member of the ADF-H (actin-depolymerizing factor-H) family of actin binding proteins. Although its predicted MW is 72 kDa, it runs anomalously at 115-116 kDa in SDS-PAGE. It is expressed by neurons, gastric Parietal cells, astrocytes, distal convoluted tubule epithelium and proton-secreting intercalated cells of the renal collecting duct. Drebrin 1 interacts with multiple partners near the membrane. It links connexin-43 and F-actin, thereby stabilizing membrane gap junctions. It also binds to EB3 (end-binding protein 3) on microtubules, facilitating actin-microtubule interactions. Human Drebrin 1 is 649 amino acids (aa) in length. It contains one actin depolymerizing homology domain (aa 3-134), an actin-binding region (≈ aa 150-300), and two HOMER binding motifs (aa 539-543 and 617-621). There are at least 10 utilized Ser/Thr phosphorylation sites and one utilized Tyr phosphorylation site. Alternative splicing generates drebrin-A (Adult), a 124-126 kDa isoform that contains a 46 aa insert after Gly319. Drebrin-A is found in neurons and possibly podocytes, and is associated with dendritic spines where it inhibits the interaction of F-actin with alpha-actinin and tropomyosin. This favors the generation of excitatory impulses in neurons. Three other potential isoform variants are noted. One utilizes an alternative start site at Met64, a second shows a 60 aa substitution for aa 1-110, and a third contains a 28 aa substitution for aa 4-29. Over aa 482-649, human Drebrin 1 shares 84% aa sequence identity with mouse Drebrin 1.

Alternate Names

D0S117E, DBN1, Drba, Drebrin A, Drebrin E, Drebrin E2

Entrez Gene IDs

1627 (Human); 56320 (Mouse); 81653 (Rat)

Gene Symbol

DBN1

UniProt

Q16643 (Human)

Additional Drebrin 1 Products

Product Documents for Human Drebrin 1 Antibody

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COA

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Product Specific Notices for Human Drebrin 1 Antibody

For research use only

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