Human E-Selectin/P-Selectin (CD62E/P) Antibody
R&D Systems, part of Bio-Techne | Catalog # BBA1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat, Porcine
Applications
Validated:
Adhesion Blockade, CyTOF-ready, Flow Cytometry, Immunocytochemistry, Immunoprecipitation
Cited:
ELISA Capture, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)
Product Specifications
Immunogen
Activated HUVEC human umbilical vein endothelial cells
Specificity
Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human E-Selectin/P-Selectin (CD62E/P) Antibody
E-Selectin (CD62E)/P-Selectin (CD62P) in HUVEC Human Cells.
E-Selectin (CD62E)/P-Selectin (CD62P) was detected in immersion fixed HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha (Catalog # 210-TA-010) using Mouse Anti-Human E-Selectin (CD62E)/P-Selectin (CD62P) Monoclonal Antibody (Catalog # BBA1) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human E-Selectin/P-Selectin (CD62E/P) Antibody
Application
Recommended Usage
Adhesion Blockade
The adhesion of U937 human histiocytic lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human P-Selectin/CD62P (Catalog # ADP3, 10 µg/mL, 100 µL/well) was maximally inhibited (80-100%) by 10 µg/mL of the antibody.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HUVEC human umbilical vein endothelial cells treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA-010)
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells activated with recombinant human TNF-alpha (Catalog # 210-TA-010)
Immunoprecipitation
Pigott, R. et al. (1991) J. Immunol. 147:130.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 3.3 using BBA1 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: E-Selectin (CD62E)/P-Selectin (CD62P)
Alternate Names
Eselectin, PSelectin
Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products
Product Documents for Human E-Selectin/P-Selectin (CD62E/P) Antibody
Product Specific Notices for Human E-Selectin/P-Selectin (CD62E/P) Antibody
For research use only
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