Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6169F

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # BBIG-E6 (13D5)

View all E-Selectin (CD62E)/P-Selectin (CD62P) Antibodies »

Product Specifications

Immunogen

Activated HUVEC human umbilical vein endothelial cells

Specificity

Detects human E‑Selectin/P‑Selectin (CD62E/P). Binds to COS cells transfected with human E-Selectin or human P-Selectin. It does not bind to CHO cells transfected with human ICAM-1, L-Selectin, PECAM-1 or VCAM-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

Detection of E-Selectin/P-Selectin (CD62E/P) antibody in HUVEC Human Umbilical Vein Endothelial Cells antibody by Flow Cytometry.

Detection of E‑Selectin/P‑Selectin (CD62E/P) in HUVEC Human Umbilical Vein Endothelial Cells by Flow Cytometry.

HUVEC human umbilical vein endothelial cells stained with TNF-a was stained with Mouse Anti-Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB6169F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HUVEC human umbilical vein endothelial cells stimulated with TNF-alpha
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: E-Selectin (CD62E)/P-Selectin (CD62P)

E-Selectin (CD62E) and P-Selectin (CD62P) are members of the Selectin/LECAM family of molecules. E- and P-Selectin are type I transmembrane glycoproteins that contain an N-terminal calcium-dependent C-type lectin domain, one EGF-like repeat, multiple sushi domains, and a transmembrane segment followed by a short cytoplasmic tail. Typically, the ligands for E-and P-Selectin are carbohydrates that contain a sialyl-Lewis X (sLeX) motif that appears on select O- and N-linked glycans and glycosphingolipids. Human E-Selectin is 95-115 kDa is size and expressed by vascular endothelium. It has a number of ligands, not all of which are shared between the human and rodent systems. For example human E-Selectin binds L-Selectin, CD44/HCELL and a poorly characterized pronase-insensitive ganglioside, none of which are recognized by mouse E-Selectin. By contrast, mouse E-Selectin binds E-Selectin Ligand whereas human E-Selectin does not. Other ligands identified for human E-Selectin include PSGL-1, CD43, Mac-1, CD66, Endoglycan and Podocalyxin-like protein. Human P-Selectin is 130-140 kDa in size, and expressed platelets and vascular endothelium. It primarily binds sLeX on O-glycans, including PSGL-1 and endoglycan. Functionally, Selectins induce leukocyte rolling on endothelial cell surfaces, an effect that activates neighboring alpha2 beta2/LFA-1 and slows leukocyte migration. Although the extracellular domains of human E- and P-Selectin show only 36% amino acid (aa) sequence identity, over the first 180 aa (out of >500 aa), E- and P- Selectin demonstrate 62% aa sequence identity.

Alternate Names

Eselectin, PSelectin

Additional E-Selectin (CD62E)/P-Selectin (CD62P) Products

Product Documents for Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human E-Selectin/P-Selectin (CD62E/P) Fluorescein-conjugated Antibody

For research use only

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