Human ENPP-1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6136A

R&D Systems, part of Bio-Techne
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FAB6136A
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Sheep IgG

View all ENPP-1 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP-2-1-2
Asp49-Trp144 (ENPP-2), Val191-Leu591 (ENPP-1), Asn532-Ile863 (ENPP-2). Cross-reactivity with human ENPP-2 was removed from the final product.
Accession # Q13822 (ENPP-2) P22413 (ENPP-1)

Specificity

Detects human ENPP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human ENPP-2 and recombinant mouse ENPP-2 is observed after removal of cross-reactivity with human ENPP-2.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human ENPP-1 APC-conjugated Antibody

Detection of ENPP-1 antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of ENPP‑1 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human ENPP-1 APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6136A, filled histogram) or isotype control antibody (Catalog # IC016A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ENPP-1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ENPP-1

Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP-1) is a transmembrane glycoprotein that hydrolyzes nucleotides and nucleotide derivatives with the formation of nucleotide-5’-monophosphates. It is inserted into the plasma membrane by an N‑terminal transmembrane domain. Human ENPP-1 has a small N-terminal cytoplasmic domain and a large C-terminal region containing two somatomedin B-like domains, a catalytic domain and a nuclease-like domain in the extracellular space (1). Defects in the ENPP-1 gene cause arterial calcification and bone mineralization abnormalities (2). ENPP-1 polymorphism or overexpression is also associated with obesity, type II diabetes and insulin resistance, which makes modulation of ENPP-1 activity one of the targets to treat insulin resistance and related diseases (1).

References

  1. Goldfine, I.D. et al. (2010) Endocrine Reviews. 29:62.
  2. Hessle, L. et al. (2002) Proc. Natl. Acad. Sci. 99:9445.
    3.  

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 1

Alternate Names

ENPP1, Ly-41 Antigen, M6S1, NPP1, NPPS, PCA1, PDNP1

Entrez Gene IDs

5167 (Human); 18605 (Mouse)

Gene Symbol

ENPP1

UniProt

Q13822 (ENPP-2) P22413 (ENPP-1)

Additional ENPP-1 Products

Product Documents for Human ENPP-1 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ENPP-1 APC-conjugated Antibody

For research use only

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