Human ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9896
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Inhibition of Cell Growth
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # H2M5B
Product Specifications
Immunogen
Human ErbB2/Her2
Specificity
Detects human ErbB2/Her2 in Western blots. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibodyTrastuzumab and uses a mouse IgG1 backbone structure. This product is for research use only.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Detection of ErbB2/Her2 in MCF-7 human cell line by Flow Cytometry.
MCF-7 human cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB9896, filled histogram) or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Inhibition of Cell Proliferation by ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Antibody.
Mouse Anti-Human ErbB2/Her2 (Based on Research Grade Trastuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9896) inhibits proliferation in the SK-BR-3 human breast cancer cell line as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 20-100 ng/mL.Applications
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: MCF-7 human cell line
Sample: MCF-7 human cell line
Inhibition of Cell Growth
Measured by its ability to inhibit proliferation in the SK‑BR‑3 human breast cancer cell line. Brodowicz, T. et al. (1997) Int. J. Cancer 73:875. The ED50 for this effect is typically 20-100 ng/mL.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ErbB2/Her2
Long Name
Receptor Tyrosine Protein Kinase ErbB2
Alternate Names
CD340, HER2, Neu Oncogene, NGL, TKR1
Gene Symbol
ERBB2
Additional ErbB2/Her2 Products
Product Specific Notices
For research use only
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