K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB49281, filled histogram) or isotype control antibody (Catalog # MAB002, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human ERMAP Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ERMAP

ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60-66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors, and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30-155) that contains one V-type Ig-like domain (aa #30-140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103-475. Over aa 30-155, human ERMAP shares 56% aa sequence identity with mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.

Long Name

Erythroblast Membrane-associated Protein

Alternate Names

RD, SC

Entrez Gene IDs

114625 (Human); 27028 (Mouse); 298485 (Rat)

Gene Symbol

ERMAP

UniProt

Q96PL5

Additional ERMAP Products

Product Documents for Human ERMAP Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ERMAP Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ERMAP Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB49281

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ERMAP Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ERMAP Antibody

Detection of ERMAP in K562 Human Cell Line by Flow Cytometry.