Human Erythropoietin R Antibody

Detection of Mouse Erythropoietin R by Western Blot

The 59 kDa protein detected by M-20 In MCF-7 cells is not bound by other anti-EpoR antibodies.The indicated lysates were immunoprecipitated (IP) then the immunoblotted (IB) with the indicated antibodies: ab10653 (abcam Inc), Mab307 (R&D systems), C-20 & M-20 (Santa Cruz Inc) or A-82 (Amgen Inc). COS cell lysates expressing a FLAG-tagged version of EpoR (FLAG-EpoR) [6] and UT-7/Epo cells served as EpoR positive controls. 769-P cells served as the EpoR negative control. (A) Westerns were immunoprecipitated (IP) with ab10653 or M-20 followed by immunoblotting (IB) with M-20. The position of full-length 59 kDa EpoR in positive controls is indicated by the arrow. Positions of molecular weight markers (kDa) are shown. Bands detected in 769-P lysates are non-EpoR cross-reacting proteins and include antibody chains that were not removed completely or protein G that leached from beads. Note the detection of a 59 kDa band with MCF-7 cells with the M:20/M:20 combination but not with the ab10653/IB:M-20 combination. (B) IP:IB combinations with the indicated antibodies were subjected to western analysis. The western slice containing the 59 kDa EpoR band from each combination is shown. Note the 59 kDa bands detected in EpoR positive controls but not 769-P cells. Only the M-20:M-20 combination detected a 59 kDa band in MCF-7 cells. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0068083), licensed under a CC-BY license. Not internally tested by R&D Systems.