Human Fetuin B Antibody

Name: Human Fetuin B Antibody
Brand: R&D Systems
SKU: MAB17251
Rating: 4 (4 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB17251

(4)

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 212607

View all Fetuin B Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fetuin B
Met19-Pro382
Accession # Q9UGM5

Specificity

Detects human Fetuin B in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhCystatin A, B, C, rhMMP-2, 9, rhBMP-2, 4, 6, rhTGF-beta 1, or rhTGF-beta 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Fetuin B Antibody

Detection of Human Fetuin B by Western Blot.

Western blot shows human serum. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Fetuin B at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Fetuin B by Simple Western^TM.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Fetuin B at approximately 62 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human Fetuin B Monoclonal Antibody (Catalog # MAB17251) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Fetuin B Antibody

Application

Recommended Usage

Simple Western

50 µg/mL
Sample: Human placenta tissue

Western Blot

5 µg/mL
Sample: Human serum

Human Fetuin B Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human Fetuin B Biotinylated Antibody (Catalog # BAM17251)
Standard: Recombinant Human Fetuin B Protein, CF (Catalog # 1725-PI)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 4.3 using MAB17251 in the following applications:

ELISA (1 Review)
Microarray (1 Review)
Microarrays (1 Review)
Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin B

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

References

Oliver, E., et al. (1999) Genomics. 57:352.
Oliver, E., et al. (2000) Biochem. J. 350:589.
Kellemann, J. et al., 1989, J. Biol. Chem. 264:14121.
Denecke, B. et al. (2003) Biochem. J. 376:135.
Schäfer, C., et al. (2003) J. Clin. Invest. 112:357.
Dziegielewska, K. M., et al. (1996) Histochem. Cell Biol. 106:319.
Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Alternate Names

FETUB

Entrez Gene IDs

26998 (Human)

Gene Symbol