Human Fetuin B Biotinylated Antibody

Name: Human Fetuin B Biotinylated Antibody
Brand: R&D Systems
SKU: BAM17251

R&D Systems, part of Bio-Techne | Catalog # BAM17251

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 212633

View all Fetuin B Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fetuin B
Met19-Pro382
Accession # NP_055190

Specificity

Detects human Fetuin B in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) Fetuin A, rhCystatin A, B, C, rhMMP-2, 9, rhBMP-2, 4, 6, rhTGF-beta 1, or rhTGF-beta 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Applications for Human Fetuin B Biotinylated Antibody

Human Fetuin B Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:

Capture Reagent: Human Fetuin B Antibody (Catalog # MAB17251)
Standard: Recombinant Human Fetuin B Protein, CF (Catalog # 1725-PI)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin B

Fetuins are members of the cystatin superfamily of cysteine protease inhibitors (1‑3). Additional members of this superfamily are kininogen and histidine-rich glycoprotein. Fetuin A and B are two known members of the fetuin family. Hepatocytes are believed to be the principal cellular source, but other cell types also express it (4, 5). Fetuin A, also known as alpha2-Heremans-Schmid glycoprotein, is an inhibitor of basic calcium phosphate precipitation and a negative acute-phase protein (6, 7). Normal circulating levels of Fetuin A in adults (300‑600 ug/mL) fall significantly (30‑50%) during injury and infection (7). Fetuin B is a newer member whose function is not fully characterized (1, 2). Fetuin A and B display similarities and differences in their characteristics. Fetuin B exhibits reduction of calcification, while both mRNA levels were down‑regulated during the acute phase in inflammation-induced rats (4). However, they share only 20% amino acid sequence identity (2). The amounts of Fetuin B in human serum, unlike Fetuin A, vary with gender and are higher in females than in males (4).

References

Oliver, E. et al. (1999) Genomics. 57:352.
Oliver, E. et al. (2000) Biochem. J. 350:589.
Kellemann, J. et al. 1989, J. Biol. Chem. 264:14121.
Denecke, B. et al. (2003) Biochem. J. 376:135.
Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Dziegielewska, K. M. et al. (1996) Histochem. Cell Biol. 106:319.
Gangneux, C. et al. (2003) Nucleic Acids Res. 31:5957.

Alternate Names

FETUB

Entrez Gene IDs

26998 (Human)

Gene Symbol