Human FGF basic/FGF2/bFGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2331
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2B Clone # 10043R
Product Specifications
Immunogen
E. coli-derived recombinant human FGF basic/FGF2/bFGF
Accession # P09038
Accession # P09038
Specificity
Detects human FGF basic/FGF2/bFGF in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody
Human FGF basic/FGF2/bFGF ELISA Standard Curve.
Recombinant Human FGF basic/FGF2/bFGF protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody(Catalog # MAB233R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2331) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human FGF basic/FGF2/bFGF Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB233R).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human FGF basic/FGF2/bFGF DuoSet ELISA Kit (Catalog # DY233) for convenient development of a sandwich ELISA or the Human FGF basic/FGF2/bFGF Quantikine ELISA Kit (Catalog # DFB50) for a complete optimized ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF basic/FGF2/bFGF
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
UniProt
Additional FGF basic/FGF2/bFGF Products
Product Documents for Human FGF basic/FGF2/bFGF Antibody
Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody
For research use only
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