Human FGF basic/FGF2/bFGF Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2333
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 10044
Product Specifications
Immunogen
E. coli-derived human FGF basic/FGF2/bFGF
Accession # P09038
Accession # P09038
Specificity
Detects human FGF basic/FGF2/bFGF in direct ELISA
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody
Detection of FGF basic/FGF2/bFGF in A172 cells (positive) and Daudi cells (negative).
FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cells (positive) and absent in Daudi human Burkitt's lymphoma cells (negative) using Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human FGF basic/FGF2/bFGF Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cells (positive) and Daudi human Burkitt's lymphoma cells (negative).
Sample: Immersion fixed A172 human glioblastoma cells (positive) and Daudi human Burkitt's lymphoma cells (negative).
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF basic/FGF2/bFGF
FGF basic, also known as FGF-2 and HBGF-2, is a member of the FGF family of mitogenic proteins. FGF basic is widely expressed and affects diverse aspects of embryonic development by regulating cell proliferation, differentiation, and survival. Various N-terminal extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. Mature human FGF basic shares greater than 96% aa sequence identity with bovine, mouse, and rat FGF basic.
Long Name
Fibroblast Growth Factor basic
Alternate Names
bFGF, FGF-2, FGF2, HBGF-2, Prostatropin
Gene Symbol
FGF2
UniProt
Additional FGF basic/FGF2/bFGF Products
Product Documents for Human FGF basic/FGF2/bFGF Antibody
Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody
For research use only
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