I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?

Name: Human FGF basic/FGF2/bFGF Antibody
Brand: R&D Systems
SKU: MAB2332

Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have several antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits , but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Human FGF basic/FGF2/bFGF Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2332

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Product Specifications
Scientific Data
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Preparation & Storage
Background
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2A Clone # 954824

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Product Specifications

Immunogen

E. coli-derived human FGF basic/FGF2/bFGF
Pro143-Ser288
Accession # P09038

Specificity

Detects Human FGF basic/FGF2/bFGF in direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2A

Scientific Data Images for Human FGF basic/FGF2/bFGF Antibody

Detection of FGF basic/FGF2/bFGF in Liver.

FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of Liver using Rat Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2332) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of FGF basic/FGF2/bFGF in A172 (Positive) & Daudi (Negative).

FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cell line (Positive) & Daudi human Burkitt's lymphoma cell line (Negative) using Rat Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2332) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to Nucleus . View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human FGF basic/FGF2/bFGF Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line (Positive) & Daudi human Burkitt's lymphoma cell line (Negative)

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF-2 and HBGF-2) is a member of the FGF superfamily of mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic are unique from other members of the family in that they lack classical secretory signal peptides. However, they are both readily secreted from cells by an alternative secretory pathway involving direct translocation and aided by several chaperones. FGF acidic (FGF-1) and FGF basic (FGF-2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. The full length human FGF basic protein is 288 amino acids, but there are multiple start sites which produce various shorter forms. Further adding to the complexity, a variety of forms of FGF basic are produced as a result of N-terminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. FGF basic has been isolated from a number of sources, including neural tissue, adrenal cortex, pituitary gland, corpus luteum, and placenta. Binding of FGF to heparin or cell surface heparan sulfate proteoglycans is required for FGF binding with high affinity to FGF receptors. FGF basic stimulates proliferation of all cells of mesodermal origin as well as many cells of neuroectodermal, ectodermal, and endodermal origin. FGF basic also induces neuronal differentiation, survival, and regeneration, and modulates embryonic development and differentiation. These observed in vitro functions suggest FGF basic may play a role in vivo in the modulation of such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally, FGF basic may also participate in the development of several pathological conditions resulting from excessive cell proliferation and/or angiogenesis.

References

Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33.
Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506.
Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

Long Name

Fibroblast Growth Factor basic

Alternate Names

bFGF, FGF-2, FGF2, HBGF-2, Prostatropin

Entrez Gene IDs

2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)

Gene Symbol

FGF2

UniProt

P09038

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Product Specific Notices for Human FGF basic/FGF2/bFGF Antibody

For research use only

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