MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5646A, filled histogram) or isotype control antibody (IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of FOLR1 in HeLa cells by Flow Cytometry

HeLa cells were stained with Mouse Anti-Human FOLR1 APC-conjugated Monoclonal Antibody (Catalog # FAB5646A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human FOLR1 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: MCF-7 human breast cancer cell line; HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1. FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk (11).

References

Kelemen, L.E. (2006) Int. J. Cancer 119:243.
Fowler, B. et al. (2001) Kidney Int. 59:S-221.
Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
Ross, J.F. et al. (1994) Cancer 73:2432.
Parker, N. et al. (2005) Anal. Biochem. 338:284.
Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Gene Symbol

FOLR1

UniProt

P15328 (Human)

Additional FOLR1 Products

Product Documents for Human FOLR1 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human FOLR1 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human FOLR1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5646A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all FOLR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human FOLR1 APC-conjugated Antibody

Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry.