Human FOLR1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB5646P

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
FAB5646P
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Knockout Validated

Cited:

Flow Cytometry, Immunocytochemistry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 548908

View all FOLR1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR1
Arg25-Met233
Accession # P15328

Specificity

Detects human FOLR1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human FOLR2, 3 or 4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human FOLR1 PE-conjugated Antibody

Detection of FOLR1 antibody in MDA-MB-231 Human Cell Line antibody by Flow Cytometry.

Detection of FOLR1 in MDA-MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of FOLR1 antibody in MCF-7 Human Cell Line antibody by Flow Cytometry.

Detection of FOLR1 in MCF-7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
FOLR1 Antibody Specificity is Shown by Flow Cytometry in Knockout Cell Line.

FOLR1 Specificity is Shown by Flow Cytometry in Knockout Cell Line.

FOLR1 knockout MCF-7 human breast cancer cell line was stained with Mouse Anti-Human FOLR1 PE-conjugated Monoclonal Antibody (Catalog # FAB5646P, filled histogram) or isotype control antibody (IC002P, open histogram). No staining in the FOLR1 knockout MCF-7 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
View 1 more images

Applications for Human FOLR1 PE-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: MDA‑MB‑231 and MCF-7 human breast cancer cell lines; HeLa human cervical epithelial carcinoma cell line

Knockout Validated

FOLR1 is specifically detected in MCF-7 human breast cancer parental cell line but is not detectable in FOLR1 knockout MCF-7 cell line.

Reviewed Applications

Read 1 review rated 4 using FAB5646P in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: FOLR1

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37‑42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3‑6). Human FOLR1 shares 83% amino acid sequence identity with mouse and rat FOLR1.  FOLR1 is predominantly expressed on epithelial cells and is dramatically up‑regulated on many carcinomas (7, 8). It is critically required during early embryogenesis as shown in knockout mice which die in utero with gross morphological defects (9). FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface (6, 10). A soluble form of FOLR1 can be proteolytically shed from the cell surface into the blood and breast milk (11).

References

  1. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  2. Fowler, B. (2001) Kidney Int. Suppl. 78:S221.
  3. Luhrs, C.A. et al. (1989) J. Biol. Chem. 264:21446.
  4. Lacey, S.W. et al. (1989) J. Clin. Invest. 84:715.
  5. Elwood, P.C. (1989) J. Biol. Chem. 264:14893.
  6. Rijnboutt, S. et al. (1996) J. Cell Biol. 132:35.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Parker, N. et al. (2005) Anal. Biochem. 338:284.
  9. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  10. Paulos, C.M. et al. (2004) Mol. Pharmacol. 66:1406.
  11. Elwood, P.C. et al. (1991) J. Biol. Chem. 26:2346.

Long Name

Folate Receptor 1

Alternate Names

FBP, Folbp1, FR-alpha, MOv18

Entrez Gene IDs

2348 (Human); 14275 (Mouse); 171049 (Rat)

Gene Symbol

FOLR1

UniProt

P15328 (Human)

Additional FOLR1 Products

Product Documents for Human FOLR1 PE-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human FOLR1 PE-conjugated Antibody

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.