Human FOLR2 Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF5697

R&D Systems, part of Bio-Techne
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BAF5697
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AF5697
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Sheep IgG

View all FOLR2 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human FOLR2
Gln22-His228
Accession # P14207

Specificity

Detects human FOLR2 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) FOLR1 and rhFOLR3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human FOLR2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human FOLR2 (Catalog # 5697-FR)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FOLR2

Folate Receptor 2 (FOLR2), also known as Folate Receptor beta, is a 38 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown (1, 2). Mature FOLR2 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage (3, 4). Human FOLR2 shares 83% amino acid sequence identity with mouse and rat FOLR2. FOLR2 is predominantly expressed in placenta, cells of the neutrophilic lineage, and some CD34+ hematopoietic progenitor cells (4‑6). It is up‑regulated on myeloid  leukemias, head and neck squamous cell carcinomas, and several nonepithelial cancers (3, 5, 7). It is also up‑regulated on macrophages and monocytes at chronic inflammatory sites including rheumatoid arthritis synovium and glioblastoma (8‑10). FOLR2 knockout mice do not show gross morphological defects, but they exhibit increased sensitivity to arsenate-induced teratogenicity (11, 12).

References

  1. Fowler, B. et al. (2001) Kidney Int. 59:S-221.
  2. Kelemen, L.E. (2006) Int. J. Cancer 119:243.
  3. Shen, F. et al. (1994) Biochemistry 33:1209.
  4. Ratnam, M. et al. (1989) Biochemistry 28:8249.
  5. Ross, J.F. et al. (1999) Cancer 85:348.
  6. Reddy, J.A. et al. (1999) Blood 93:3940.
  7. Ross, J.F. et al. (1994) Cancer 73:2432.
  8. Nakashima-Matsushita, N. et al. (1999) Arthritis Rheum. 42:1609.
  9. van der Heijden, J.W. et al. (2009) Arthritis Rheum. 60:12.
  10. Nagai, T. et al. (2009) Cancer Immunol. Immunother. Feb 24 epub.
  11. Piedrahita, J.A. et al. (1999) Nat. Genet. 23:228.
  12. Wlodarczyk, B. et al. (2001) Toxicol. Appl. Pharmacol. 177:238.

Long Name

Folate Receptor 2

Alternate Names

beta-HFR, FR-beta, FR-P3

Entrez Gene IDs

2350 (Human); 14276 (Mouse); 293154 (Rat)

Gene Symbol

FOLR2

UniProt

P14207 (Human)

Additional FOLR2 Products

Product Documents for Human FOLR2 Biotinylated Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human FOLR2 Biotinylated Antibody

For research use only

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