Human FoxC1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6329
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 625905
Product Specifications
Immunogen
E. coli-derived recombinant human FoxC1
Gln208-Gln322
Accession # Q12948
Gln208-Gln322
Accession # Q12948
Specificity
Detects human FoxC1 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human FoxC1 Antibody
Detection of Human FoxC1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, SH-SY5Y human neuroblastoma cell line, NCI-H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human FoxC1 Monoclonal Antibody (Catalog # MAB6329) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FoxC1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human FoxC1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, NCI‑H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line
Sample: HeLa human cervical epithelial carcinoma cell line, SH‑SY5Y human neuroblastoma cell line, NCI‑H460 human large cell lung carcinoma cell line, and HEK293 human embryonic kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxC1
Human FoxC1 (forkhead box C1), also called FKHL7 (forkhead-like 7) or FREAC3 (forkhead-related activator 3) is a 553 amino acid (aa) intracellular phosphoprotein that belongs to a large family of nuclear transcription factors that share a common forkhead/winged helix DNA binding domain. FoxC1 is essential for formation of mesodermal tissues; mutations underlie Axenfeld-Rieger malformations of the eye. The human FoxC1 sequence includes a DNA binding domain at aa 77‑168 and an inhibitory domain between aa 268‑245. Phosphorylation within this inhibitory region causes a protein gel mobility shift. Human FoxC1 shares 92% and 91% aa identity with mouse and rat FoxC1, respectively.
Long Name
Forkhead Box C1
Alternate Names
ARA, FKHL7, FREAC3, IGDA, IHG1, IRID1, RIEG3
Gene Symbol
FOXC1
UniProt
Additional FoxC1 Products
Product Documents for Human FoxC1 Antibody
Product Specific Notices for Human FoxC1 Antibody
For research use only
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