Human GDF-1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6937

R&D Systems, part of Bio-Techne
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MAB6937
MAB6937-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 748450

View all GDF-1 Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GDF-1
Asp254-Arg372
Accession # NP_001483

Specificity

Detects human GDF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant mouse (rm) GDF-1 is observed, and no cross-reactivity with recombinant human (rh) GDF-3 is observed. In Western blots, no cross-reactivity with rhGDF-3 or rmGDF-7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GDF-1 Antibody

Detection of Human GDF-1 antibody by Western Blot.

Detection of Human GDF‑1 by Western Blot.

Western blot shows lysates of human brain (cortex) tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GDF-1 Monoclonal Antibody (Catalog # MAB6937) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GDF-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human GDF-1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human brain (cortex) tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-1

Growth and Differentiation Factor 1 (GDF‑1) is a secreted protein in the TGF‑ beta superfamily (1). It is the functional equivalent of frog, zebrafish, and chick Vg1 (2, 3). Human GDF‑1 is synthesized as a preproprotein that produces an approximately 44 kDa proprotein after cleavage of a 29 amino acid (aa) signal peptide. Further proteolysis at a dibasic cleavage site produces a 224 aa propeptide and a secreted 119 aa, 12‑15 kDa mature peptide (4). Mature human GDF‑1 shares 81% aa sequence identity with mouse and rat GDF‑1. GDF‑1 is expressed in the developing and adult nervous system, particularly in neurons of the cerebral cortex and hippocampus (4, 5). It is required during embryogenesis for development of left/right axis asymmetry, anterior/posterior axis asymmetry, and the notochord (2, 3, 6, 7). It cooperates with Nodal and GDF‑3 in these activities and potentiates Nodal bioactivity through formation of a 27 kDa noncovalent GDF‑1‑Nodal heterodimer (3, 7, 8). The presence of Nodal is required for GDF‑1 induced effects (8). GDF‑1 signals through heteromeric receptor complexes containing Activin RIIA or Activin RIIB, ALK4 or ALK7, and an EGF‑CFC protein such as Cryptic or Cripto (7‑9). Loss of function mutations in GDF‑1 are associated with a variety of human cardiac development defects (10).

References

  1. Rider, C.C. and B. Mulloy (2010) Biochem. J. 429:1.
  2. Wall, N.A. et al. (2000) Dev. Biol. 227:495.
  3. Andersson, O. et al. (2007) Dev. Biol. 311:500.
  4. Lee, S.-J. (1991) Proc. Natl. Acad. Sci. 88:4250.
  5. Soderstrom, S. and T. Ebendal (1999) J. Neurosci. Res. 56:482.
  6. Rankin, C.T. et al. (2000) Nat. Genet. 24:262.
  7. Andersson, O. et al. (2006) Dev. Biol. 293:370.
  8. Tanaka, C. et al. (2007) Genes Dev. 21:3272.
  9. Cheng, S.K. et al. (2003) Genes Dev. 17:31.
  10. Karkera, J.D. et al. (2007) Am. J. Hum. Genet. 81:987.

Long Name

Growth Differentiation Factor 1

Alternate Names

GDF1

Entrez Gene IDs

2657 (Human); 14559 (Mouse)

Gene Symbol

GDF1

UniProt

NP_001483

Additional GDF-1 Products

Product Documents for Human GDF-1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human GDF-1 Antibody

For research use only

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