HEK293 human embryonic kidney cell line transfected with human GHSR and eGFP was stained with either (A) Mouse Anti-Human GHSR Monoclonal Antibody (Catalog # MAB8370) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Applications for Human GHSR Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 human embryonic kidney cell line transfected with human GHSR and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHSR

GHSR is a member of the G protein-coupled receptor family that may play a role in energy homeostasis and regulation of body weight. Two conserved transcript variants for GHSR are expressed in several tissues. This transcript, 1a, excises an intron and encodes the functional protein. GHSR is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. Mutations in GHSR are associated with autosomal idiopathic short stature.

Long Name

Growth Hormone Secretagogue Receptor

Alternate Names

GH-releasing peptide receptor, Ghrelin receptor, GHRP, GHS-R, growth hormone secretagogue receptor, growth hormone secretagogue receptor type 1

Entrez Gene IDs

2693 (Human); 208188 (Mouse); 84022 (Rat)

Gene Symbol

GHSR

UniProt

NP_940799

Additional GHSR Products

Product Documents for Human GHSR Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human GHSR Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human GHSR Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8370

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all GHSR Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human GHSR Antibody

Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry.