Human GIPR Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8210
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Blockade of Receptor-ligand Interaction, CyTOF-ready, Flow Cytometry, Immunohistochemistry
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 591853
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human GIPR.
Met1-Cys466
Accession # P48546
Met1-Cys466
Accession # P48546
Specificity
Detects human GIPR in direct ELISAs. Stains human GIPR transfected cells but not irrelevant transfectants.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human GIPR Antibody
Detection of GIPR in HEK293 Human Cell Line Transfected with Human GIPR and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human GIPR or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.
GIPR in Human Pancreas.
GIPR was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islet cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Human GIP binding to human GIPR-transfected HEK293 cells blocked by Human GIPR Antibody.
In a functional flow cytometry test, biotinylated recombinant Human GIP (Catalog # 2257, 25 ng/mL) binds to Human GIPR-transfected HEK293 cells (black dotted line). Binding is completely blocked (orange histogram) by 2.5 µg/mL of Mouse Anti-Human GIPR Monoclonal Antibody (Catalog # MAB8210). Mouse IgG1Isotype Control (Catalog # MAB002) at 2.5 µg/mL was used as a control (blue line). Cells were stained with Sterptavidin-APC (Catalog # F0050).Applications for Human GIPR Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
At 2.5 ug/mL, this antibody will block >95% of the binding.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with human GIPR and eGFP
Sample: HEK293 human embryonic kidney cell line transfected with human GIPR and eGFP
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human pancreas subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human pancreas subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 3 using MAB8210 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GIPR
Long Name
Gastric Inhibitory Polypeptide Receptor
Alternate Names
PGQTL2
Gene Symbol
GIPR
UniProt
Additional GIPR Products
Product Documents for Human GIPR Antibody
Product Specific Notices for Human GIPR Antibody
For research use only
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