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Human Granzyme K Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # IC10216T

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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IC10216T-100

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Rabbit IgG Clone # 2471A

Product Specifications

Immunogen

Synthetic peptide containing human Granzyme K
Accession # P49863

Specificity

Detects human Granzyme K in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Granzyme K Alexa Fluor® 594-conjugated Antibody

Detection of Granzyme K by Flow Cytometry.

NK cells treated with Recombinant Human IL-2 Protein (202-IL), Recombinant Human IL-12 Protein (219-IL), Recombinant Human IL-18/IL-1F4 Protein (9124-IL) and Recombinant Human IL-21 Protein (8879-IL) for 10 days were stained with Rabbit Anti-Human NCAM-1/CD56 Alexa Fluor® 647-conjugated Monoclonal Antibody (FAB24086R) and either (A) Rabbit Anti-Human Granzyme K Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC10216T) or (B) Normal Rabbit IgG Control (AB-105-C). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human Granzyme K Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

Titration recommended for optimal concentration with starting range of 0.1-1 µg/1 million cells. Sample used for this experiment were day 10 expanded NK cells.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Granzyme K

Granzymes are released by cytoplasmic granules within NK and cytotoxic T cells.  They are serine proteases that induce apoptosis in the target cell.  Granzymes have also been found to help initiate the inflammatory response by activating macrophages and mast cells when in an extracellular state.  Granzymes have also been found to protect the body against the formation of different kinds of lymphomas.

References

  1. Bots, M. and JP Medema (2006). J.Cell Sci. 119:5011.
  2. Walch, M. et al. (2014). Cell. 157:1309.
  3. Cullen, SP. et al. (2010). Cell Death Differ. 17:616.

Alternate Names

Fragmentin-3, Granzyme 3, Granzyme-K, GZMK, NK-Tryp-2, NK-Tryptase-2, TRYP2, Tryptase II

Entrez Gene IDs

3003 (Human); 14945 (Mouse); 29165 (Rat)

Gene Symbol

GZMK

UniProt

Additional Granzyme K Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Granzyme K Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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