Human GRP78/HSPA5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4846
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Fish - Danio rerio (Zebrafish)
Applications
Validated:
Simple Western, Western Blot
Cited:
Immunocytochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 474421
Product Specifications
Immunogen
E. coli-derived recombinant human GRP78
Met1-Leu654
Accession # P11021
Met1-Leu654
Accession # P11021
Specificity
Detects endogenous human GRP78 at 78 kDa in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GRP78/HSPA5 Antibody
Detection of Human GRP78/HSPA5 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRP78/HSPA5 at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human GRP78/HSPA5 by Simple WesternTM.
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GRP78/HSPA5 at approximately 72 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human GRP78/HSPA5 Monoclonal Antibody (Catalog # MAB4846). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.*Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.Applications for Human GRP78/HSPA5 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB4846 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRP78/HSPA5
GRP78 (Glucose-regulated protein 78 kDa; also BiP and HSPA5) is a 72 kDa member of the heat shock protein 70 family of proteins. Intracellularly, GRP78 is an endoplasmic reticulum chaperone that participates in protein folding; extracellularly, it induces IL-10 production from T cells and interacts with Cripto to block TGF-beta signaling. Human GRP78 precursor is 654 amino acids (aa) in length. It contains an 18 aa signal sequence and a 636 aa mature region that shows a hydantoinase A region (aa 145‑245) and a C-terminal KDEL motif that is present on intracellular GRP78, but absent on secreted GRP78. There is alternative splicing in the signal sequence (aa 1‑10), and multiple single aa substitituion. Over aa 1‑654, human GRP78 is more than 97% aa identical to mouse and rat GRP78.
Long Name
75 KD Glucose Regulated Protein
Alternate Names
BIP, HSP70-5, HSPA5
Gene Symbol
HSPA5
UniProt
Additional GRP78/HSPA5 Products
Product Documents for Human GRP78/HSPA5 Antibody
Product Specific Notices for Human GRP78/HSPA5 Antibody
For research use only
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