Human GSK-3 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2986
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 288901
Product Specifications
Immunogen
E. coli-derived recombinant human GSK-3 alpha
Gly90-Ser483
Accession # P49840.2
Gly90-Ser483
Accession # P49840.2
Specificity
Detects human GSK-3 alpha. In direct ELISAs, this antibody does not detect recombinant human GSK-3 beta.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human GSK-3 alpha Antibody
GSK‑3 alpha in MCF‑7 Human Cell Line.
GSK-3a was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human GSK-3a Monoclonal Antibody (Catalog # MAB2986) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human GSK-3 alpha Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GSK-3 alpha
Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta) share 85% amino acid identity. GSK-3 alpha is inhibited by phosphorylation at S21 by Akt and other kinases. Deregulated GSK-3 has been implicated in several diseases including type II diabetes, Alzheimer’s disease, bipolar disorder, and cancer.
Long Name
Glycogen Synthase Kinase 3 alpha
Alternate Names
GSK3 alpha, GSK3A
Gene Symbol
GSK3A
UniProt
Additional GSK-3 alpha Products
Product Documents for Human GSK-3 alpha Antibody
Product Specific Notices for Human GSK-3 alpha Antibody
For research use only
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