Human HES-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3600
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 455237
Product Specifications
Immunogen
E. coli-derived recombinant human HES-4
Val94-Arg221
Accession # Q9HCC6
Val94-Arg221
Accession # Q9HCC6
Specificity
Detects human HES-4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human HES-4 Antibody
Detection of Human HES-4 by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line. Gels were loaded with 30 µg of whole cell lysate (WCL), 20 µg of cytoplasmic (Cyto), and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human HES-4 Monoclonal Antibody (Catalog # MAB3600) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for HES-4 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.Applications for Human HES-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: PC-3 human prostate cancer cell line
Sample: PC-3 human prostate cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB3600 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HES-4
The Notch pathway maintains embryonic stem cells by up‑regulation of HES and Hey basic helix-loop-helix family members, which repress tissue specific expression. HES-4 is up‑regulated in gastric and colorectal cancers. It shares high homology with HES-1, HES-2, and HES-3.
Long Name
Hairy And Enhancer of Split 4
Alternate Names
HES4
Entrez Gene IDs
57801 (Human)
Gene Symbol
HES4
UniProt
Additional HES-4 Products
Product Documents for Human HES-4 Antibody
Product Specific Notices for Human HES-4 Antibody
For research use only
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