Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human HLA Class I PE-conjugated Antibody

Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PE-conjugated Monoclonal Antibody (Catalog # FAB7098P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HLA Class I PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Reviewed Applications

Read 2 reviews rated 4.5 using FAB7098P in the following applications:

Flow Cytometry (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA Class I

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

References

Barnstable, C.J. et al. (1978) Cell 14:9.

Long Name

Class I Human Leukocyte Antigen

Alternate Names

HLA-A, HLA-ABC

Entrez Gene IDs

3105 (Human)

Gene Symbol

HLA-A

Additional HLA Class I Products

Product Documents for Human HLA Class I PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human HLA Class I PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human HLA Class I PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7098P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications