Detects the human major histocompatibility complex (MHC) class I, HLA-A, B, and C. Recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both the HLA molecule and beta 2-Microglobulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human HLA Class I PerCP-conjugated Antibody

Detection of HLA Class I in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Mouse Anti-Human HLA Class I PerCP-conjugated Monoclonal Antibody (Catalog # FAB7098C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human HLA Class I PerCP-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood lymphocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: HLA Class I

The MHC (Major Histocompatibility Complex) is a group of at least 200 genes located on chromosome 6 in human. It contains multiple groupings, one of which is called Class I that contains three distinct, but closely related molecules. These three molecules are known as MHC Class I-A, -B, and -C which, in the human, have been renamed HLA (Human Leukocyte Antigen)-A, -B, and -C. All are 44-46 kDa type I transmembrane glycoproteins that share approximately 85% amino acid sequence identity in their extracellular domains. And all represent the alpha- component of a alpha- beta heterodimer that utilizes the 11-12 kDa transmembrane beta2-microglobulin protein as a beta-component. These HLA heterodimers appear on all nucleated cells, and serve as a platform for the presentation of cytoplasmic components (both self and foreign) to the alpha beta-TCR of cytotoxic CD8+ T cells. Unedited or mutated "self" components should be ignored, while tumor or viral components should elicit a cytotoxic immune response. This requires the continuous internal "processing" or degradation of large proteins into 8-10 amino acid peptides that are subsequently bound to a type A, B or C heterodimer and cycled to the plasma membrane. On the cell surface, the B chain is most common while the C chain is least common. And with advancing age, both the A and B chains decline in number. Not all A, B and C chains are "engaged"; while 90% of the HLA B chains are associated with antigen, only 30-70% of A and C chains are associated with processed antigens. And identical peptides can be perceived differently. For instance, a nine amino acid peptide with O-linked (but not N-linked) glycosylation will not be recognized by a CD8+ T cell that is specific for the naked nine amino acid peptide. Finally, the A, B and C chains, if not the entire heterodimeric complex, are now known to act in-cis with LILRB2, generating an activating complex on select cell types. The mouse MHC counterpart to the human HLA system is called H-2, and the two mouse genes that correspond to human HLA-A and -B show 68% amino acid sequence identity over their entire lengths.

Long Name

Class I Human Leukocyte Antigen

Alternate Names

HLA-A, HLA-ABC

Entrez Gene IDs

3105 (Human)

Gene Symbol

HLA-A

Additional HLA Class I Products

Product Documents for Human HLA Class I PerCP-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human HLA Class I PerCP-conjugated Antibody

For research use only

Citations
Reviews

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Flow Cytometry

Human HLA Class I PerCP-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7098C

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all HLA Class I Antibodies »

Product Specifications

Immunogen

Specificity