Human Hormone-sensitive Lipase/HSL Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7104
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 729923
Product Specifications
Immunogen
E. coli-derived recombinant human Hormone-sensitive Lipase/HSL
Met302-Leu425
Accession # Q05469
Met302-Leu425
Accession # Q05469
Specificity
Detects human Hormone-sensitive Lipase/HSL in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Hormone-sensitive Lipase/HSL Antibody
Detection of Human and Mouse Hormone-sensitive Lipase/HSL by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, NTera-2 human testicular embryonic carcinoma cell line, SVEC4-10 mouse vascular endothelial cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Hormone-sensitive Lipase/HSL at approximately 70 kDa and 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hormone-sensitive Lipase/HSL in Human Testis.
Hormone-sensitive Lipase/HSL was detected in immersion fixed paraffin-embedded sections of human testis using Mouse Anti-Human Hormone-sensitive Lipase/HSL Monoclonal Antibody (Catalog # MAB7104) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of spermatocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human Hormone-sensitive Lipase/HSL Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human testis
Sample: Immersion fixed paraffin-embedded sections of human testis
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line, NTera‑2 human testicular embryonic carcinoma cell line, SVEC4‑10 mouse vascular endothelial cell line, and HT‑29 human colon adenocarcinoma cell line
Sample: MCF‑7 human breast cancer cell line, NTera‑2 human testicular embryonic carcinoma cell line, SVEC4‑10 mouse vascular endothelial cell line, and HT‑29 human colon adenocarcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Hormone-sensitive Lipase/HSL
Alternate Names
Hormonesensitive Lipase, HSL, LHS, LIPE
Gene Symbol
LIPE
UniProt
Additional Hormone-sensitive Lipase/HSL Products
Product Documents for Human Hormone-sensitive Lipase/HSL Antibody
Product Specific Notices for Human Hormone-sensitive Lipase/HSL Antibody
For research use only
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