Human HOXA1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5014
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HOXA1
Met1-Asp118
Accession # P49639
Met1-Asp118
Accession # P49639
Specificity
Detects human HOXA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) HOXB1, rhHOXB4, and rhHOXA9 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human HOXA1 Antibody
HOXA1 in NTera-2 Human Cell Line.
HOXA1 was detected in immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line using 10 µg/mL Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human HOXA1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HOXA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5014) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HOXA1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human HOXA1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line
Sample: Immersion fixed retinoic acid-treated NTera-2 human testicular embryonic carcinoma cell line
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line
Sample: A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HOXA1
HOXA1 (Homeobox A1; also Hox-1F) is a 35 kDa member of the Antp homeobox family of transcription factors. It is expressed in epithelium and is upregulated by E‑cadherin signaling, whereupon it suppresses apoptosis and promotes anchorage dependent growth. Human HOXA1 is 335 amino acids (aa) in length. It contains two poly-His sequences (aa 65-74 and 142-146), a PBX binding segment (aa 204-209) and a DNA binding homeobox domain (aa 229-288). There are multiple splice variants. There is a potential alternate start site at Met6 that may be paired with a nine aa substitution for the C‑terminal 117 aa, or a 19 aa substitution for the C‑terminal 217 amino acids. Over aa 1-118, human HOXA1 shares 92% and 90% aa identity with mouse and canine HOXA1, respectively.
Long Name
Homeobox A1
Alternate Names
BSAS, HOX1F
Gene Symbol
HOXA1
UniProt
Additional HOXA1 Products
Product Documents for Human HOXA1 Antibody
Product Specific Notices for Human HOXA1 Antibody
For research use only
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