Human IFITM2/IFITM3 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4834
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived human IFITM3
His3-His57
Accession # Q01628
His3-His57
Accession # Q01628
Specificity
Detects human IFITM2 and human IFITM3 in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human IFITM1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IFITM2/IFITM3 Antibody
Detection of Human IFITM2/IFITM3/Fragilis by Western Blot.
Western blot shows lysates of human brain tissue and human spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IFITM2/3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4834) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for IFITM2 and IFITM3 at approximately 15 and 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human IFITM2/IFITM3 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Human brain tissue and human spleen tissue
Sample: Human brain tissue and human spleen tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFITM2/IFITM3
IFITM3 (IFN-inducible tranemembrane protein 3; also IFN-inducible protein 1-8U and fragilis) is a 14.6 kDa (predicted) member of the CD225 family of proteins. It is expressed on primordial germ cells and select tumor types, and appears to regulate cell migration. Human IFITM3 is a 133 amino acid (aa), two-transmembrene protein that contains an N-terminal (aa 1-57) and a C-terminal (aa 108-128) extracellular domain. Over aa 3-57, human IFITM3 is 59% and 56% aa identical to bovine and mouse IFITM3, respectively. Over aa 3-57 human IFITM3 is 88% identical to human IFITM2.
Alternate Names
1-8D;1-8U;DSPA2b;DSPA2c;IFITM2;IFITM3;IP15
UniProt
Additional IFITM2/IFITM3 Products
Product Documents for Human IFITM2/IFITM3 Antibody
Product Specific Notices for Human IFITM2/IFITM3 Antibody
For research use only
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