Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human IFN-alpha / beta R1 Monoclonal Antibody (Catalog # MAB2452) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IFN-alpha / beta R1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of IFN-alpha / beta R1 in U937 cells by Flow Cytometry.

U937 cells were stained with Rabbit Anti-Human IFN-alpha / beta R1 Monoclonal Antibody (Catalog # MAB2452, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IFN-alpha / beta R1 Antibody

Application

Recommended Usage

Flow Cytometry

0.10 µg/10⁶ cells
Sample: U937 human histiocytic lymphoma cell line

Western Blot

2 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha/beta R1

Interferon‑alpha/beta receptor 1 (IFN‑ alpha/ beta R1), also known as IFNAR1, is a 100‑130 kDa member of the class II cytokine receptor family of proteins. These proteins form heterodimeric receptor complexes that mediate class II cytokine signals. Subunits of the different receptor complexes are shared and serve multiple functions (1). IFN‑ alpha/ beta R1, in association with IFN‑ alpha/ beta R2, is required for propagating anti‑microbial signal transduction triggered by the type 1 interferons such as IFN‑ alpha and IFN‑ beta (2, 3). Mature human IFN‑ alpha/ beta R1 consists of a 409 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 100 aa cytoplasmic domain (4). The ECD contains three tandem fibronectin type III repeats and is extensively glycosylated. Within the ECD, human IFN‑ alpha/ beta R1 shares 47% and 50% aa identity with mouse and rat IFN‑ alpha/ beta R1, respectively. Alternative splicing generates two additional isoforms that lack the transmembrane segment and either all or a portion of the cytoplasmic domain. IFN‑ alpha/ beta R1 interacts very weakly or not at all with type 1 interferons and does not stably interact with IFN‑ alpha/ beta R2. Ligands preferentially associate with IFN‑ alpha/ beta R2, and this complex subsequently forms a stable ternary assembly with IFN‑ alpha/ beta R1 (5‑7). IFN‑ alpha/ beta R1 also associates with IFN‑ gamma R2 even in the absence of IFN‑ gamma stimulation (3). IFN‑ alpha/ beta R1 activation depends on tyrosine phoshorylation as well as palmitoylation of its cytoplasmic domain (8, 9). Rapid down‑regulation of the receptor is accomplished by ligand‑dependent or ‑independent pathways (e.g. VEGF R signaling, TLR signaling, or cellular stress) which induce its serine phosphorylation, ubiquitination, and degradation (10‑13).

References

Langer, J.A. et al. (2004) Cytokine Growth Factor Rev. 15:33.
Hwang, S.Y. et al. (1995) Proc. Natl. Acad. Sci. USA 92:11284.
Takaoka, A. et al. (2000) Science 288:2357.
Uze, G. et al. (1990) Cell 60:225.
Lamken, P. et al. (2004) J. Mol. Biol. 341:303.
Arduini, R.M. et al. (1999) Prot. Sci. 8:1867.
Kalie, E. et al. (2008) J. Biol. Chem. 283:32925.
Platanias, L.C. (2005) Nat. Rev. Immunol. 5:375.
Claudinon, J. et al. (2009) J. Biol. Chem. 284:24328.
Zheng, H. et al. (2011) Blood 118:4003.
Qian, J. et al. (2011) PLoS Pathogens 7:e1002065.
Bhattacharya, S. et al. (2010) J. Biol. Chem. 285:2318.
Bhattacharya, S. et al. (2011) J. Biol. Chem. 286:22069.

Long Name

Interferon alpha/beta Receptor 1

Alternate Names

IFN-aR1, IFN-bR1, IFNAR1, IFNbR1

Entrez Gene IDs

3454 (Human); 15975 (Mouse); 101926190 (Cynomolgus Monkey)

Gene Symbol

IFNAR1

UniProt

P17181

Additional IFN-alpha/beta R1 Products

Product Documents for Human IFN-alpha / beta R1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

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Product Specific Notices for Human IFN-alpha / beta R1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human IFN-alpha / beta R1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2452

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all IFN-alpha/beta R1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human IFN-alpha / beta R1 Antibody

Detection of Human IFN-alpha / beta R1 by Western Blot.