Human IgG3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10122
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 985438
Product Specifications
Immunogen
Purified human IgG3
Specificity
Detects human IgG3 in direct ELISAs and Western blots. In Western blots, no cross-recativity with human IgG1, human IgG2, and human IgG4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human IgG3 Antibody
Detection of Human IgG3by Western Blot.
Western blot shows lysates of purified human IgG1(negative control), purified human IgG2(negative control), purified human IgG3, and human purified human IgG4(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG3Monoclonal Antibody (Catalog # MAB10122) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG3at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IgG3 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Purified human IgG3
under non-reducing conditions only
Sample: Purified human IgG3
under non-reducing conditions only
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG3
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G3
Alternate Names
DKFZp686H11213, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, IgG3, immunoglobulin heavy constant gamma 3 (G3m marker), MGC45809
Additional IgG3 Products
Product Documents for Human IgG3 Antibody
Product Specific Notices for Human IgG3 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...