Human/Primate IgG Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1101
Key Product Details
Species Reactivity
Human, Primate
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1268B
Product Specifications
Immunogen
Human IgG
Specificity
Detects human and primate IgG in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Primate IgG Antibody
Detection of Human and Primate IgG by Western Blot.
Western blot shows human serum, primate, mouse, and rat serum. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for human and primate IgG at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human IgG by Simple WesternTM.
Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for IgG at approximately 60 kDa (as indicated) using 2 µg/mL of Rabbit Anti-Human/Primate IgG Monoclonal Antibody (Catalog # MAB1101) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Primate IgG Antibody
Application
Recommended Usage
Simple Western
2 µg/mL
Sample: Human serum
Sample: Human serum
Western Blot
0.05 µg/mL
Sample: Human serum and primate serum
Sample: Human serum and primate serum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Long Name
Immunoglobulin G
Alternate Names
Immunoglobulin G, ImmunoglobulinG
Additional IgG Products
Product Documents for Human/Primate IgG Antibody
Product Specific Notices for Human/Primate IgG Antibody
For research use only
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