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Human IgG Alexa Fluor® 647-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB110R

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 97924

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region

Specificity

Detects chimeric proteins containing a human IgG Fc region in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IgG Alexa Fluor® 647-conjugated Antibody

Detection of IgG Fc antibody in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP antibody by Flow Cytometry.

Detection of IgG Fc in Mouse B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Mouse PD-1 and eGFP by Flow Cytometry.

(A) Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7) bound to HEK293 human embryonic kidney cell line transfected with mouse PD-1 and eGFP or (B) cells alone, were stained with Mouse Anti-Human IgG Fc Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB110R). Quadrants were set based on Mouse IgG1 Alexa Fluor® 647 Isotype Control (Catalog # IC002R, data not shown). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IgG Alexa Fluor® 647-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25-1 µg/106 cells
Sample: Recombinant Mouse B7‑H1/PD‑L1 Fc Chimera (Catalog # 1019-B7) bound to HEK293 human embryonic kidney cell line transfected with mouse PD-1 and eGFP

Reviewed Applications

Read 1 review rated 5 using FAB110R in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human IgG Alexa Fluor® 647-conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IgG Alexa Fluor® 647-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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